HIGH RANCHU MON

HIGH RANCHU MON
Art by Alexander Vasiljev, Copyright © 2020

HIGH RANCHU STORE - AZAYAKA RANCHU FOOD

Thursday, August 17, 2017

ATTEMPT AT CLONING RANCHU

I have stumbled upon a very interesting paper published in Zygote, Volume 20, Issue 1, February, 2012, pp.79-85, Cambridge University Press.

Attempt at cloning high-quality goldfish breed ‘Ranchu’ by fin-cultured cell nuclear transplantation

Daisuke Tanaka, Akito Takahashi, Akinori Takai, Hiromi Ohta and Koichi Ueno

Summary:

The viability of ornamental fish culture relies on the maintenance of high-quality breeds. To improve the profitability of culture operations we attempted to produce cloned fish from the somatic nucleus of the high-quality Japanese goldfish (Carassius auratus auratus) breed ‘Ranchu’. We transplanted the nucleus of a cultured fin-cell from an adult Ranchu into the non-enucleated egg of the original goldfish breed ‘Wakin’. Of the 2323 eggs we treated, 802 underwent cleavage, 321 reached the blastula stage, and 51 reached the gastrula stage. Two of the gastrulas developed until the hatching stage. A considerable number of nuclear transplants retained only the donor nucleus. Some of these had only a 2n nucleus derived from the same donor fish. Our results provide insights into the process of somatic cell nuclear transplantation in teleosts, and the cloning of Ranchu.


Vocabulary:

Cleavage - is the division of cells in the early embryo.
Blastula stage - is a hollow sphere of cells, referred to as blastomeres, surrounding an inner fluid-filled cavity called the blastocoele formed during an early stage of embryonic development in animals.
Gastrula stage - early stage during which the single-layered blastula is reorganized into a multilayered structure.
Teleost - a large group comprising all ray-finned fishes.


2017 © Alexander Vasiljev


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